Coding

Part:BBa_M45077:Design

Designed by: Yessica Castro   Group: UtahState_BE5930_-_S14   (2014-04-09)

Heavy Metal Binding Protein (hmbp) gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 82


Design Notes

The start codon of the nucleotide sequence was initially gcc that codes for Alanine (Ala). The start codon was changed to Methionite (atg).

Source

HMBP gene was acquired from a genetic DNA library of activated-sludge microorganisms .

References

Sano D., Myojo K. and Omura T., Cloning of a heavy-metal-binding protein derived from activated sludge microorganisms, Applied and Environ. Microbiol., 72(9),6377-6380 (2006).